cd55  (R&D Systems)

Journal: International Journal of Molecular Sciences

Article Title: Complement-Mediated Two-Step NETosis: Serum-Induced Complement Activation and Calcium Influx Generate NADPH Oxidase-Dependent NETs in Serum-Free Conditions

doi: 10.3390/ijms25179625

Figure Lengend Snippet: Complement activation increases complement regulators on neutrophils. Neutrophils were precoated with anti-CD59 monoclonal antibodies and then exposed to normal human serum (NHS) to activate the complement cascade (complement stimulated). The group not treated with anti-CD59 monoclonal antibodies served as the unstimulated control (serum). The cells were then fixed, immunostained, and analyzed by flow cytometry. The expression level of all three complement regulators (CD46, CD55, and CD59) was significantly increased on complement-activated neutrophils compared to the unstimulated controls. ( A ) Representative flow cytometry tracings of an experiment; ( B ) mean fluorescence intensity (MFI) for each marker from all experiments. n = 3 biological replicates. * p < 0.05, compared to their controls, based on the paired t-test. Data are presented as means ± SD.

Article Snippet: To specifically deactivate the complement activity of serum, while leaving other serum proteins functional, serum was heated for 56 degrees Celsius for 30 min. Primary antibodies C3b (1:500; Mouse, ThermoFisher Invitrogen, Carlsbad, CA, USA; Cat# MA1-70054), C5b-9 (1:250, Rabbit, Abcam, Toronto, ON, Canada; Cat# Ab55811), CD46 (1:200, Rabbit, Santa Cruz Biotech, Dallas, TX, USA; Cat# SC 9098), CD55 (1:250, Goat, R&D Systems, 614 McKinley Place NE, Minneapolis, MN, USA; Cat# AF2009), CD59 (1:200, Rabbit ThermoFisher Invitrogen, Carlsbad, CA, USA; Cat# PA5-34513), and PE CD11b (BioLegend, Markham, ON, Canada; Cat# 301306) and were incubated for 30 min on dark ice and then rinsed twice with spins at 400 g for 10 min.

Techniques: Activation Assay, Control, Flow Cytometry, Expressing, Fluorescence, Marker

Journal: World Journal of Surgical Oncology

Article Title: CD55 may be an important prognostic factor of thymic epithelial tumors: a retrospective study

doi: 10.1186/s12957-025-04051-2

Figure Lengend Snippet: Expression of CD55 mRNA and protein in normal thymus, thymoma, and TSCC. The expression levels of CD55 mRNA ( A ) and protein ( B ) were significantly elevated progressively in normal thymus tissue, thymoma, and TSCC

Article Snippet: The primary rabbit anti-human CD55 polyclonal antibody (Proteintech, USA) was diluted at a ratio of 1:1400.

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: CD55 may be an important prognostic factor of thymic epithelial tumors: a retrospective study

doi: 10.1186/s12957-025-04051-2

Figure Lengend Snippet: Intensities of CD55 expression in TETs (IHC) scored 0: negative expression ( A , 40×; a, 200×); scored 1: low expression ( B , 40×; b, 200×); scored 2: moderate expression ( C , 40×; c, 200×); scored 3: high expression ( D , 40×; d, 200×)

Article Snippet: The primary rabbit anti-human CD55 polyclonal antibody (Proteintech, USA) was diluted at a ratio of 1:1400.

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: CD55 may be an important prognostic factor of thymic epithelial tumors: a retrospective study

doi: 10.1186/s12957-025-04051-2

Figure Lengend Snippet: Proportion of CD55 expression in TETs (IHC) scored 0: negative expression ( A , 40×; a, 200×); scored 1: 1% − 30% positive cells ( B , 40×; b, 200×); scored 2: 30% − 60% positive cells ( C , 40×; c, 200×); scored 3: ≥ 60% positive cells ( D , 40×; d, 200×)

Article Snippet: The primary rabbit anti-human CD55 polyclonal antibody (Proteintech, USA) was diluted at a ratio of 1:1400.

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: CD55 may be an important prognostic factor of thymic epithelial tumors: a retrospective study

doi: 10.1186/s12957-025-04051-2

Figure Lengend Snippet: Kaplan-Meier Survival Analysis in TETs The results demonstrated that PFS of TETs was closely related to CD55 mRNA ( A , p < 0.0001), CD55 protein ( B , p < 0.0001), tumor completeness of resection ( C , p < 0.0001), histological type ( D , p < 0.0001), Masaoka-Koga stage ( E , p < 0.0001), and adjuvant therapy ( F , p = 0.0009), but no with gender ( G , p = 0.1805) or MG ( H , p = 0.7269)

Article Snippet: The primary rabbit anti-human CD55 polyclonal antibody (Proteintech, USA) was diluted at a ratio of 1:1400.

Techniques: Adjuvant

Journal: World Journal of Surgical Oncology

Article Title: CD55 may be an important prognostic factor of thymic epithelial tumors: a retrospective study

doi: 10.1186/s12957-025-04051-2

Figure Lengend Snippet: The relationship between CD55 and the clinical pathological features of patients The expression levels of CD55 mRNA and protein were higher in R1/R2 group ( A ), higher histological grade ( B ), Masaoka-Koga stage III/IV ( C ), adjuvant therapy ( D ) compared with the corresponding R0 group, normal thymus tissues, and stage I/II, and no-adjuvant therapy. Moreover, the expression of CD55 protein was significantly higher expressed in ≥ 60 years compared with ˂60 years ( E ). Additionally, CD55 protein expression was higher in males than that in females ( F ), whereas CD55 mRNA did not show similar results ( E, F ). Patients without MG showed higher CD55 mRNA and protein expression levels than those with MG, however, this difference was not statistically significant ( G ). There was also no statistically significant relationship between tumor diameter and CD55 expression ( H ). When CD55 protein was highly expressed, the mRNA level was also high ( I ).

Article Snippet: The primary rabbit anti-human CD55 polyclonal antibody (Proteintech, USA) was diluted at a ratio of 1:1400.

Techniques: Expressing, Adjuvant

Journal: World Journal of Surgical Oncology

Article Title: CD55 may be an important prognostic factor of thymic epithelial tumors: a retrospective study

doi: 10.1186/s12957-025-04051-2

Figure Lengend Snippet: Kaplan-Meier Survival Analysis in high-risk TETs The results demonstrated that PFS of high-risk TETs was closely related to high expression of CD55 mRNA (A, p < 0.0001) and CD55 protein (B, p = 0.0168)

Article Snippet: The primary rabbit anti-human CD55 polyclonal antibody (Proteintech, USA) was diluted at a ratio of 1:1400.

Techniques: Expressing